| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ERAB aa 1-200. |
| Positive control: | A549 (Human lung adenocarcinoma cell) cell lysate, U-87 MG (Human glioblastoma cell) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q99714 Human | O70351 Rat |
| Alternative names: | 17 beta hydroxysteroid dehydrogenase 10 17 beta hydroxysteroid dehydrogenase type 10 17-beta-HSD 10 17-beta-hydroxysteroid dehydrogenase 10 17b HSD10 3 hydroxy 2 methylbutyryl CoA dehydrogenase 3 hydroxyacyl CoA dehydrogenase type 2 3 hydroxyacyl CoA dehydrogenase type II 3-hydroxy-2-methylbutyryl-CoA dehydrogenase 3-hydroxyacyl-CoA dehydrogenase type II 3-hydroxyacyl-CoA dehydrogenase type-2 AB binding alcohol dehydrogenase ABAD Ads9 Amyloid beta binding polypeptide Amyloid beta peptide binding alcohol dehydrogenase Amyloid beta peptide binding protein Amyloid beta peptide binding protein CAMR DUPXp11.22 Endoplasmic Reticulum Amyloid Binding Protein Endoplasmic reticulum associated amyloid beta peptide binding protein Endoplasmic reticulum-associated amyloid beta-peptide-binding protein ER associated amyloid beta-binding protein ERAB HADH 2 HADH2 HCD 2 HCD2 HCD2_HUMAN Hsd17b10 Hydroxyacyl CoA Dehydrogenase type II Hydroxy...... |
|
Fig1:
Western blot analysis of ERAB on different lysates with Rabbit anti-ERAB antibody (HA500150) at 1/5,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: U-87 MG (Human glioblastoma cell) cell lysate Lane 3: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 4: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 5: C6 (Rat glioma cell) cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500150, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 27 kDa Observed band size: 27 kDa |