ERAB Rabbit Polyclonal Antibody
cat.: HA500150
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ERAB aa 1-200. |
| Positive control: | A549 cell lysate, MCF-7 cell lysate, rat kidney tissue, human tonsil tissue, human liver tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q99714 Human | O70351 Rat |
| Alternative names: | 17 beta hydroxysteroid dehydrogenase 10 17 beta hydroxysteroid dehydrogenase type 10 17-beta-HSD 10 17-beta-hydroxysteroid dehydrogenase 10 17b HSD10 3 hydroxy 2 methylbutyryl CoA dehydrogenase 3 hydroxyacyl CoA dehydrogenase type 2 3 hydroxyacyl CoA dehydrogenase type II 3-hydroxy-2-methylbutyryl-CoA dehydrogenase 3-hydroxyacyl-CoA dehydrogenase type II 3-hydroxyacyl-CoA dehydrogenase type-2 AB binding alcohol dehydrogenase ABAD Ads9 Amyloid beta binding polypeptide Amyloid beta peptide binding alcohol dehydrogenase Amyloid beta peptide binding protein Amyloid beta peptide binding protein CAMR DUPXp11.22 Endoplasmic Reticulum Amyloid Binding Protein Endoplasmic reticulum associated amyloid beta peptide binding protein Endoplasmic reticulum-associated amyloid beta-peptide-binding protein ER associated amyloid beta-binding protein ERAB HADH 2 HADH2 HCD 2 HCD2 HCD2_HUMAN Hsd17b10 Hydroxyacyl CoA Dehydrogenase type II Hydroxy...... |
Images
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Fig1:
Western blot analysis of ERAB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500150, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ERAB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500150, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ERAB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500150, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERAB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500150, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.