ASIC4 Rabbit Polyclonal Antibody
cat.: HA500151
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ASIC4 aa 250-450. |
| Positive control: | HepG2 cell lysate, K562 cell lysate, rat brain tissue, mouse pituitary tissue, SH-SY5Y. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q96FT7 Human | Q7TNS7 Mouse | Q9JHS6 Rat |
| Alternative names: | Acid-sensing ion channel 4 ASIC4 Amiloride-sensitive cation channel 4 Amiloride-sensitive cation channel 4, pituitary ACCN4 |
Images
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Fig1:
Western blot analysis of ASIC4 on different lysates with Rabbit anti-ASIC4 antibody (HA500151) at 1/5,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 110 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500151, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size:59 kDa Observed band size:70 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ASIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500151, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue using anti-ASIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500151, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.