Glucosidase 2 subunit beta Rabbit Polyclonal Antibody
cat.: HA500152
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 59 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human Glucosidase 2 subunit beta aa 30-230.
Positive control: Hela cell lysate, Jurkat cell lysate, A431 cell lysate, rat kidney tissue lysate, rat liver tissue lysate, human liver tissue, human kidney tissue, Hela, SiHa.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:2,000
1:200
1:100-1:500
1:500-1:1,000
Uniprot #: SwissProt: P14314 Human
Alternative names: 80K-H protein AGE-binding receptor 2 AGE-R2 G19P1 GLU2B_HUMAN Glucosidase 2 subunit beta Glucosidase II beta subunit Glucosidase II subunit beta Hepatocystin PCLD PKCSH PLD1 PRKCSH Protein kinase C substrate 60.1 kDa protein heavy chain Protein kinase C substrate 80 Kda protein Protein kinase C substrate 80K-H Protein kinase C substrate, 80 Kda protein
Images
HA500152_1.jpg Fig1: Western blot analysis of Glucosidase 2 subunit beta on different lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (HA500152) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate

Lysates/proteins at 10 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 75 kDa

Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500152) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500152_2.jpg Fig2: Western blot analysis of Glucosidase 2 subunit beta on HeLa (Human cervical adenocarcinoma cell) lysates with Rabbit anti-Glucosidase 2 subunit beta antibody (HA500152) at 1/5,000 dilution.

Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500152, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 59 kDa
Observed band size: 80 kDa
HA500152_3.jpg Fig3: Immunocytochemistry analysis of SiHa cells labeling Glucosidase 2 subunit beta with Rabbit anti-Glucosidase 2 subunit beta antibody (HA500152) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Glucosidase 2 subunit beta antibody (HA500152) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA500152_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucosidase 2 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500152, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500152_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucosidase 2 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500152, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500152_6.jpg Fig6: Flow cytometric analysis of Hela cells labeling Glucosidase 2 subunit beta.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500152, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.