GNB3 Rabbit Polyclonal Antibody
cat.: HA500153
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNB3 aa 10-60. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, rat kidney tissue, mouse brain tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P16520 Human | Q61011 Mouse | P52287 Rat |
| Alternative names: | G protein beta 3 subunit GBB3_HUMAN GNB3 GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 GTP binding regulatory protein beta 3 chain Guanine nucleotide binding protein (G protein) beta polypeptide 3 Guanine nucleotide binding protein G Guanine nucleotide binding protein G(I)/G(S)/G(T) beta subunit 3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 Hypertension associated protein IG SG Transducin beta chain 3 Tsubunit beta 3 |
Images
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Fig1:
Western blot analysis of GNB3 on different lysates with Rabbit anti-GNB3 antibody (HA500153) at 1/5,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500153, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/20,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
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Fig2:
Western blot analysis of GNB3 on different lysates with Rabbit anti-GNB3 antibody (HA500153) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 2minutes6seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500153, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500153, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500153, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.