GNB3 Rabbit Polyclonal Antibody
cat.: HA500153
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNB3 aa 10-60. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, mouse brain tissue lysate, human brain tissue lysate, rat brain tissue lysate, rat kidney tissue, mouse brain tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P16520 Human | Q61011 Mouse | P52287 Rat |
| Alternative names: | G protein beta 3 subunit GBB3_HUMAN GNB3 GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 GTP binding regulatory protein beta 3 chain Guanine nucleotide binding protein (G protein) beta polypeptide 3 Guanine nucleotide binding protein G Guanine nucleotide binding protein G(I)/G(S)/G(T) beta subunit 3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 Hypertension associated protein IG SG Transducin beta chain 3 Tsubunit beta 3 |
Images
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Fig1:
Western blot analysis of GNB3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500153, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Human brain tissue lysate Lane 5: Rat brain tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500153, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GNB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500153, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.