RBBP7 Rabbit Polyclonal Antibody
cat.: HA500155
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RBBP7 aa 225-425. |
| Positive control: | HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, C6 cell lysate, PC-12 cell lysate, human cervix tissue, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:100-1:400 |
| Uniprot #: | SwissProt: Q16576 Human | Q60973 Mouse | Q71UF4 Rat |
| Alternative names: | G1/S transition control protein binding protein RbAp46 Histone acetyltransferase type B subunit 2 Histone binding protein RBBP7 Histone-binding protein RBBP7 MGC138867 MGC138868 Nucleosome remodeling factor subunit RBAP46 Nucleosome-remodeling factor subunit RBAP46 RBAP46 RBBP 7 RBBP-7 RBBP7 RBBP7_HUMAN Retinoblastoma binding protein 7 Retinoblastoma binding protein p46 Retinoblastoma-binding protein 7 Retinoblastoma-binding protein p46 Retinoblastoma-binding protein RbAp46 |
Images
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Fig1:
Western blot analysis of RBBP7 on different lysates with Rabbit anti-RBBP7 antibody (HA500155) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: COS-1 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: F9 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 52 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500155) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-RBBP7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500155, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-RBBP7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500155, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.