CDKN1A/P21 Rabbit Polyclonal Antibody
cat.: HA500156
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.5ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CDKN1A/P21 aa 1-164. |
| Positive control: | MCF7 treated with 10μM NUTLIN 3A for 24 hours cell lysate, U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P38936 Human |
| Alternative names: | CAP20 CDK-interacting protein 1 CDKI CDKN1 Cdkn1a CDN1A_HUMAN CIP1 Cyclin Dependent Kinase Inhibitor 1A Cyclin-dependent kinase inhibitor 1 Cyclin-dependent kinase inhibitor 1A (P21) Cyclin-dependent kinase inhibitor 1A (p21, Cip1) DNA Synthesis Inhibitor MDA-6 MDA6 Melanoma differentiation-associated protein 6 Melanoma differentiation-associated protein p21 P21 protein p21CIP1 p21Cip1/Waf1 p21WAF PIC1 SDI1 SLC12A9 WAF1 Wild type p53 activated fragment 1 (WAF1) Wild type p53 activated fragment 1 Wildtype p53-activated fragment 1 |
Images
|
Fig1:
Western blot analysis of CDKN1A/P21 on different lysates with Rabbit anti-CDKN1A/P21 antibody (HA500156) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated with 10μM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500156) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CDKN1A/P21 on different lysates with Rabbit anti-CDKN1A/P21 antibody (HA500156) at 1/2,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500156) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: MCF7 (Human breast cancer cell) treated with or without 10μM Nutlin 3a for 24 hours Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA500156, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.