Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 24 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human MAD4 aa 1-180. |
Positive control: | Mouse brain tissue lysate, mouse lung tissue lysate. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB |
1:1,000-1:2,000 |
Uniprot #: | SwissProt: Q14582 Human | Q60948 Mouse |
Alternative names: | bHLHc12 Class C basic helix loop helix protein 12 Class C basic helix-loop-helix protein 12 MAD 4 MAD4 Mad4 homolog MAD4_HUMAN Max associated protein 4 MAX dimerization protein 4 Max dimerizer 4 Max interacting transcriptional repressor MAD4 Max-associated protein 4 Max-interacting transcriptional repressor MAD4 MST 149 MST149 MSTP 149 MSTP149 MXD 4 MXD4 |
Fig1:
Western blot analysis of MAD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500157, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Mouse lung tissue lysate |