PAR1 Rabbit Polyclonal Antibody
cat.: HA500159
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PAR1 aa 158-176 (Extracellular domain). |
| Positive control: | 293T (Human embryonic kidney cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, Rat kidney tissue lysate, Mouse kidney tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P25116 Human | P30558 Mouse | P26824 Rat |
| Alternative names: | CF2R Coagulation factor II (thrombin) receptor Coagulation factor II receptor F2R HTR PAR 1 PAR-1 PAR1 PAR1_HUMAN Protease activated receptor 1 Proteinase activated receptor 1 Proteinase-activated receptor 1 Thrombin receptor TR |
Images
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Fig1:
Western blot analysis of PAR1 on different lysates with Rabbit anti-PAR1 antibody (HA500159) at 1/2,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500159, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 36/40 kDa |
|
Fig2:
Western blot analysis of PAR1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500159, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat kidney tissue lysate Lane 2: Mouse kidney tissue lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.