PPP2R1A Rabbit Polyclonal Antibody
cat.: HA500160
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPP2R1A aa 100-400. |
| Positive control: | Hela cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, rat smooth muscle tissue. |
| Subcellular location: | Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: P30153 Human | Q76MZ3 Mouse |
| Alternative names: | 2AAA_HUMAN 6330556D22Rik Alpha isoform of regulatory subunit A, protein phosphatase DKFZp470L0914 Medium tumor antigen-associated 61 kDa protein MGC128399 MGC786 MGC83000 MGC95083 PP2A Aalpha PP2A PP2A subunit A isoform PR65-alpha PP2A subunit A isoform R1-alpha PP2A, subunit A, PR65-alpha isoform PP2A, subunit A, R1-alpha isoform PP2AAALPHA Ppp2r1a ppp2r1a-b PR65 PR65-alpha PR65A Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a Protein phosphatase 2 (formerly 2A), regulatory subunit A, Protein phosphatase 2 regulatory subunit A alpha Protein phosphatase 2, 65-KD regulatory subunit A, alpha Protein phosphatase 2, regulatory subunit A, alpha isoform Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform |
Images
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Fig1:
Western blot analysis of PPP2R1A on different lysates with Rabbit anti-PPP2R1A antibody (HA500160) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate, 10 µg/Lane Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, 10 µg/Lane Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate, 10 µg/Lane Lane 4: Mouse cerebellum tissue lysate, 20 µg/Lane Lane 5: C6 (Rat glioma cell) cell lysate, 10 µg/Lane Lane 6: Rat brain tissue lysate, 20 µg/Lane Exposure time: 24 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500160, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 65.3 kDa Observed band size: 65 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-PPP2R1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500160, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.