Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 79 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human DDX4 aa 1-200. |
Positive control: | Mouse testis tissue lysate, rat testis tissue lysate. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000-1:2,000 |
Uniprot #: | SwissProt: Q9NQI0 Human | Q61496 Mouse | Q64060 Rat |
Alternative names: | DDX 4 Ddx4 DDX4_HUMAN DEAD (Asp Glu Ala Asp) box polypeptide 4 DEAD box protein 4 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 MVH Probable ATP dependent RNA helicase DDX4 Probable ATP-dependent RNA helicase DDX4 VASA Vasa homolog |
Fig1:
Western blot analysis of DDX4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500161, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate |