Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Rat |
Applications: | WB, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 14 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human COX5B aa 50-90. |
Positive control: | 293 cell lysates, rat brain tissue, human liver tissue, human liver carcinoma tissue, human small intestine tissue, HepG2. |
Subcellular location: | Mitochondrion inner membrane. |
Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
Uniprot #: | SwissProt: P10606 Human | P12075 Rat |
Alternative names: | COX 5B COX5B COX5B_HUMAN COXVB Cytochrome c oxidase polypeptide Vb Cytochrome c oxidase polypeptide VB mitochondrial Cytochrome c oxidase subunit 5B Cytochrome c oxidase subunit 5B mitochondrial Cytochrome c oxidase subunit Vb mitochondrial |
Fig1: Western blot analysis of COX5B on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500163, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. | |
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-COX5B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500163, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-COX5B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500163, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-COX5B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500163, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-COX5B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500163, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |