Flightless 1 Rabbit Polyclonal Antibody
cat.: HA500167
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 145 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Flightless 1 aa 1100-1269 / 1269. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, human tonsil tissue, human lung tissue, mouse lung tissue, rat testis tissue. |
| Subcellular location: | Cytoskeleton, centrosome, nucleus, focal adhesion. |
| Recommended Dilutions:
WB IHC-P |
1:10,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q13045 Human | Q9JJ28 Mouse Entrez Gene: 287375 Rat |
| Alternative names: | Fli 1 FLI Fli1 Flightless 1 Flightless I (Drosophila) homolog Flightless I homolog Flightless I homolog (Drosophila) Flightless1 FlightlessI fliI FLII_HUMAN Fliih FLIL MGC39265 Protein flightless 1 homolog Protein flightless-1 homolog |
Images
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Fig1:
Western blot analysis of Flightless 1 on different lysates with Rabbit anti-Flightless 1 antibody (HA500167) at 1/10,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lane 4: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500167, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 145 kDa Observed band size: 145 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Flightless 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Flightless 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Flightless 1 antibody (HA500167) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Flightless 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.