ENAH Rabbit Polyclonal Antibody
cat.: HA500168
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ENAH aa 100-150. |
| Positive control: | SiHa cell lysate, 293T cell lysate, human breast carcinoma tissue, mouse testis tissue, SiHa. |
| Subcellular location: | Cytoskeleton, cytoplasm, lamellipodium, filopodium, synapse, focal adhesion. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:200-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8N8S7 Human | Q03173 Mouse |
| Alternative names: | ENA Enah ENAH_HUMAN MENA NDPP1 Protein enabled homolog |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ENAH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500168, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ENAH antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500168, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Flow cytometric analysis of ENAH was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500168, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig4:
Western blot analysis of ENAH on different lysates with Rabbit anti-ENAH antibody (HA500168) at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cells) cell lysate Lane 1: MDA-MB-231 (Human breast cancer cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500168, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 66.5 kDa Observed band size: 67 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.