Spry2 Rabbit Polyclonal Antibody
cat.: HA500170
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Spry2 aa 1-180. |
| Positive control: | Rat liver tissue lysates, human liver tissue. |
| Subcellular location: | Ruffle membrane, cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:200 |
| Uniprot #: | SwissProt: O43597 Human Entrez Gene: 306141 Rat |
| Alternative names: | hSPRY 2 hSPRY2 MGC23039 Protein sprouty homolog 2 Sprouty 2 Sprouty homolog 2 (Drosophila) Sprouty homolog 2 Sprouty2 Spry 2 Spry-2 SPRY2 SPY2_HUMAN |
Images
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Fig1:
Western blot analysis of Spry2 on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500170, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 35 kDa Observed band size: 35 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Spry2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500170, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.