| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 138 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EVI1 aa 1-200. |
| Positive control: | 293 cell lysates, A431 cell lysates. |
| Subcellular location: | Nucleus, Nucleus speckle, Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q03112 Human |
| Alternative names: | AML1 EVI 1 AML1 EVI 1 fusion protein AML1 EVI1 Ecotropic viral integration site 1 Ecotropic virus integration 1 site protein Ecotropic virus integration site 1 protein homolog EVI 1 EVI1 MDS1 and EVI1 complex locus MDS1 and EVI1 complex locus protein EVI1 MDS1 and EVI1 complex locus protein MDS1 MDS1 MDS1 EVI1 MGC163392 MGC97004 Myelodysplasia syndrome 1 Myelodysplasia syndrome 1 protein Myelodysplasia syndrome-associated protein 1 Oncogene EVI1 PRDM3 Zinc finger protein Evi1 |
|
Fig1:
Western blot analysis of EVI1 on different lysates with Rabbit anti-EVI1 antibody (HA500173) at 1/2,000 dilution. Lane 1: SK-OV-3(Human ovarian cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500173, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 138 kDa Observed band size: 138 kDa |