Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 107/138 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Evi-1 aa 1-200. |
Positive control: | 293 cell lysates, A431 cell lysates. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: Q03112 Human |
Alternative names: | AML1 EVI 1 AML1 EVI 1 fusion protein AML1 EVI1 Ecotropic viral integration site 1 Ecotropic virus integration 1 site protein Ecotropic virus integration site 1 protein homolog EVI 1 EVI1 MDS1 and EVI1 complex locus MDS1 and EVI1 complex locus protein EVI1 MDS1 and EVI1 complex locus protein MDS1 MDS1 MDS1 EVI1 MGC163392 MGC97004 Myelodysplasia syndrome 1 Myelodysplasia syndrome 1 protein Myelodysplasia syndrome-associated protein 1 Oncogene EVI1 PRDM3 Zinc finger protein Evi1 |
Fig1: Western blot analysis of EVI1 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500173, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. | |
Fig2: Western blot analysis of EVI1 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500173, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |