EVI1 Rabbit Polyclonal Antibody
cat.: HA500173
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 138 kDa
Isotype: IgG
Immunogen: Recombinant protein within human EVI1 aa 1-200.
Positive control: 293 cell lysates, A431 cell lysates.
Subcellular location: Nucleus, Nucleus speckle, Cytoplasm.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q03112 Human
Alternative names: AML1 EVI 1 AML1 EVI 1 fusion protein AML1 EVI1 Ecotropic viral integration site 1 Ecotropic virus integration 1 site protein Ecotropic virus integration site 1 protein homolog EVI 1 EVI1 MDS1 and EVI1 complex locus MDS1 and EVI1 complex locus protein EVI1 MDS1 and EVI1 complex locus protein MDS1 MDS1 MDS1 EVI1 MGC163392 MGC97004 Myelodysplasia syndrome 1 Myelodysplasia syndrome 1 protein Myelodysplasia syndrome-associated protein 1 Oncogene EVI1 PRDM3 Zinc finger protein Evi1
Images
HA500173_1.jpg Fig1: Western blot analysis of EVI1 on different lysates with Rabbit anti-EVI1 antibody (HA500173) at 1/2,000 dilution.

Lane 1: SK-OV-3(Human ovarian cancer cell) cell lysate


Lysates/proteins at 20 µg/Lane.
Exposure time: 180 seconds; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500173, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 138 kDa
Observed band size: 138 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.