SF3A1 Rabbit Polyclonal Antibody
cat.: HA500175
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SF3A1 aa 744-793. |
| Positive control: | HL-60 cell lysate, Daudi cell lysate, Hela cell lysate, 293T cell lysate, mouse ovary tissue, Hela, mouse brian tissue, mouse lung tissue. |
| Subcellular location: | Nucleus, Nucleus speckle. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q15459 Human | Q8K4Z5 Mouse |
| Alternative names: | Pre mRNA processing 21 Pre mRNA splicing factor SF3a PRP21 PRPF21 SAP 114 SAP114 sf3a1 SF3A1_HUMAN SF3a120 Spliceosome associated protein 114 Spliceosome-associated protein 114 Splicing factor 3 subunit 1 Splicing factor 3a subunit 1 120kDa Splicing factor 3A subunit 1 |
Images
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Fig1:
Western blot analysis of SF3A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500175, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lane 3: Hela cell lysate Lane 4: 293T cell lysate Predicted band size: 89 kDa Observed band size: 120/50/55 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-SF3A1 antibody (HA500175) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500175) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of Hela cells labeling SF3A1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500175, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brian tissue with Rabbit anti-SF3A1 antibody (HA500175) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SF3A1 antibody (HA500175) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.