ATR Rabbit Polyclonal Antibody
cat.: HA500176
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 301 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 1198-1410. |
| Positive control: | Hela cell lysates, rat large intestine tissue. |
| Subcellular location: | Nucleus, PML body, Chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q13535 Human | D3Z822 Rat |
| Alternative names: | Ataxia telangiectasia and Rad3 related Ataxia telangiectasia and Rad3-related protein ATR ATR_HUMAN FCTCS FRAP Related Protein 1 FRAP-related protein 1 FRP1 MEC1 MEC1 mitosis entry checkpoint 1 homolog Protein kinase ATR RAC3 Rad3 related protein SCKL SCKL1 Serine/threonine protein kinase ATR Serine/threonine-protein kinase ATR |
Images
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Fig1: Western blot analysis of ATR on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500176, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-ATR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500176, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.