IL-32 Rabbit Polyclonal Antibody
cat.: HA500179
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 27 kDa
Isotype: IgG
Immunogen: Recombinant protein within human IL-32 aa 20-180.
Positive control: Jurkat cell lysates, SW620, human liver tissue, human breast carcinoma tissue, human pancreas tissue, MCF-7, SiHa.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:100-1:200
1:500-1:2,000
1:200-1:500
Uniprot #: SwissProt: P24001 Human
Alternative names: IL 32alpha IL 32beta IL 32delta IL 32gamma IL-32 IL32 IL32_HUMAN Interleukin 32 Interleukin 32 small Interleukin 32 theta Interleukin-32 interleukin-32 eta Natural killer cell transcript 4 Natural killer cells protein 4 NK 4 NK4 OTTHUMP00000236040 OTTHUMP00000236041 OTTHUMP00000236042 OTTHUMP00000236043 OTTHUMP00000236044 OTTHUMP00000236045 OTTHUMP00000236046 OTTHUMP00000236047 OTTHUMP00000236048 OTTHUMP00000236049 OTTHUMP00000236050 OTTHUMP00000236051 OTTHUMP00000236052 OTTHUMP00000236053 OTTHUMP00000236054 OTTHUMP00000241545 OTTHUMP00000241602 OTTHUMP00000241603 OTTHUMP00000241604 OTTHUMP00000241605 OTTHUMP00000241606 OTTHUMP00000241607 OTTHUMP00000241608 OTTHUMP00000241609 OTTHUMP00000241610 OTTHUMP00000241611 OTTHUMP00000241612 TAIF TAIFa TAIFb TAIFc TAIFd Tumor necrosis factor alpha-inducing factor
Images
HA500179_1.jpg Fig1: Western blot analysis of IL-32 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500179, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
HA500179_2.jpg Fig2: ICC staining of IL-32 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500179, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500179_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL-32 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500179, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500179_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IL-32 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500179, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500179_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IL-32 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500179, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500179_6.jpg Fig6: Flow cytometric analysis of IL-32 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500179, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA500179_7.jpg Fig7: Immunocytochemistry analysis of SiHa cells labeling IL-32 with Rabbit anti-IL-32 antibody (HA500179) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-32 antibody (HA500179) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.