2B4 Rabbit Polyclonal Antibody
cat.: HA500180
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human 2B4 aa 30-60. |
| Positive control: | Human spleen tissue, human tonsil tissue. |
| Subcellular location: | Cell membrane, Membrane. |
| Recommended Dilutions:
IHC-P |
1:200-1:1,000 |
| Uniprot #: | SwissProt: Q9BZW8 Human |
| Alternative names: | 2B4 C9.1 CD244 CD244 antigen CD244 molecule CD244 molecule natural killer cell receptor 2B4 CD244 natural killer cell receptor 2B4 CD244_HUMAN F730046O15Rik h2B4 Ly90 NAIL Natural killer cell activation-inducing ligand Natural killer cell receptor 2B4 NK cell activation inducing ligand NK cell activation inducing ligand NAIL NK cell activation-inducing ligand NK cell type I receptor protein 2B4 NKR2B4 Nmrk Non-MHC restricted killing associated OTTHUMP00000027884 p38 signaling lymphocytic activation molecule 4 SLAM family, member 4 SLAMF4 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-2B4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500180, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-2B4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500180, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.