PRSS2 Rabbit Polyclonal Antibody
cat.: HA500182
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, IHC-P, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PRSS2 aa 1-120.
Positive control: Human small intestine tissue lysates, rat pancreas tissue, human pancreas tissue, mouse pancreas tissue, SH-SY5Y.
Subcellular location: Extracellular space.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:500-1:1,000
1:200-1:1,000
1:50-1:200
Uniprot #: SwissProt: P07478 Human | P07146 Mouse | P00763 Rat
Alternative names: Anionic trypsinogen EC 3.4.21.4 MGC111183 MGC120174 Protease serine 2 preproprotein Protease, serine, 2 (trypsin 2) Protease, serine, 2 Protease, serine, 2, preproprotein Prss2 Serine protease 2 TRY2 TRY2_HUMAN TRY8 TRYP2 Trypsin 2 Trypsin II Trypsin-2 Trypsinogen 2
Images
HA500182_1.jpg Fig1: Western blot analysis of PRSS2 on human small intestine tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500182, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500182_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-PRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500182, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500182_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500182, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500182_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-PRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500182, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500182_5.jpg Fig5: ICC staining of PRSS2 in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500182, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.