PRSS2 Rabbit Polyclonal Antibody
cat.: HA500182
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PRSS2 aa 1-120. |
| Positive control: | Human small intestine tissue lysates, rat pancreas tissue, human pancreas tissue, mouse pancreas tissue, SH-SY5Y. |
| Subcellular location: | Extracellular space. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:200-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P07478 Human | P07146 Mouse | P00763 Rat |
| Alternative names: | Anionic trypsinogen EC 3.4.21.4 MGC111183 MGC120174 Protease serine 2 preproprotein Protease, serine, 2 (trypsin 2) Protease, serine, 2 Protease, serine, 2, preproprotein Prss2 Serine protease 2 TRY2 TRY2_HUMAN TRY8 TRYP2 Trypsin 2 Trypsin II Trypsin-2 Trypsinogen 2 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-PRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500182, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500182, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-PRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500182, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: ICC staining of PRSS2 in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500182, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Western blot analysis of PRSS2 on human small intestine lysate with Rabbit anti-PRSS2 antibody (HA500182) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500182, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 26.5 kDa Observed band size: 26 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.