KAT4/TAF1 Rabbit Polyclonal Antibody
cat.: HA500183
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Cow, Chicken, Dog
Applications: WB, IHC-P, IF, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 215 kDa
Isotype: IgG
Immunogen: Recombinant protein within human aa 1500-1700.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF
  ELISA

1:500-1:2,000
1:100-1:500
1:100-1:500
1:5,000-1:10,000
Uniprot #: SwissProt: P21675 Human | Q80UV9 Mouse
Entrez Gene: 317256 Rat
Alternative names: BA2R CCG 1 CCG1 CCGS Cell cycle G1 phase defect Cell cycle gene 1 protein Complementation of cell cycle block G1 to S DYT3 protein NSCL 2 NSCL2 OF p250 TAF 1 TAF(II)250 TAF1 RNA polymerase II TAF130 TAF145 TAF2A TAFII 250 TAFII250 TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD TATA box binding protein (TBP)-associated factor TBP associated factor 1 TBP associated factor 145 kDa TBP associated factor 250 kDa Transcription factor TFIID p250 polypeptide Transcription initiation factor TFIID 250 kDa subunit Transcription initiation factor TFIID subunit 1 YGR274C
Images
HA500183_1.jpg Fig1: Sample:
Lane 1: Human 293T cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-KAT4/TAF1 (HA500183) at 1/1,000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution
Predicted band size: 215 kDa
Observed band size: 300 kDa
HA500183_2.jpg Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KAT4/TAF1) Polyclonal Antibody, Unconjugated (HA500183) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HA500183_3.jpg Fig3: Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (KAT4/TAF1) Polyclonal Antibody, Unconjugated (HA500183) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HA500183_4.jpg Fig4: Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (KAT4/TAF1) Polyclonal Antibody, Unconjugated (HA500183) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.