TAF1 Rabbit Polyclonal Antibody
cat.: HA500183
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cow, Chicken, Dog |
| Applications: | WB, IHC-P, IF, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 215 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 1500-1700. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF ELISA |
1:500-1:2,000 1:100-1:500 1:100-1:500 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P21675 Human | Q80UV9 Mouse Entrez Gene: 317256 Rat |
| Alternative names: | BA2R CCG 1 CCG1 CCGS Cell cycle G1 phase defect Cell cycle gene 1 protein Complementation of cell cycle block G1 to S DYT3 protein NSCL 2 NSCL2 OF p250 TAF 1 TAF(II)250 TAF1 RNA polymerase II TAF130 TAF145 TAF2A TAFII 250 TAFII250 TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD TATA box binding protein (TBP)-associated factor TBP associated factor 1 TBP associated factor 145 kDa TBP associated factor 250 kDa Transcription factor TFIID p250 polypeptide Transcription initiation factor TFIID 250 kDa subunit Transcription initiation factor TFIID subunit 1 YGR274C |
Images
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Fig1:
Sample: Lane 1: Human 293T cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-TAF1 (HA500183) at 1/1,000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 215 kDa Observed band size: 300 kDa |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TAF1) Polyclonal Antibody, Unconjugated (HA500183) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig3: Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TAF1) Polyclonal Antibody, Unconjugated (HA500183) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig4: Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (TAF1) Polyclonal Antibody, Unconjugated (HA500183) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.