TAF1 Rabbit Polyclonal Antibody
cat.: HA500183
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 213 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 1500-1700. |
| Positive control: | Rat kindey tissue, mouse colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P21675 Human | Q80UV9 Mouse Entrez Gene: 317256 Rat |
| Alternative names: | BA2R CCG 1 CCG1 CCGS Cell cycle G1 phase defect Cell cycle gene 1 protein Complementation of cell cycle block G1 to S DYT3 protein NSCL 2 NSCL2 OF p250 TAF 1 TAF(II)250 TAF1 RNA polymerase II TAF130 TAF145 TAF2A TAFII 250 TAFII250 TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD TATA box binding protein (TBP)-associated factor TBP associated factor 1 TBP associated factor 145 kDa TBP associated factor 250 kDa Transcription factor TFIID p250 polypeptide Transcription initiation factor TFIID 250 kDa subunit Transcription initiation factor TFIID subunit 1 YGR274C |
Images
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Fig1:
Western blot analysis of TAF1 on TAF1 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500183) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions Lane 2: HDG11 recombinant protein with primary antibody at 1:5,000 dilutions |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kindey tissue using anti-TAF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500183, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TAF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500183, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.