EZH1 Rabbit Polyclonal Antibody
cat.: HA500184
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 86/81/80/69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EZH1 aa 500-747. |
| Positive control: | HepG2 cell lysate, mouse hippocampusl tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q92800 Human | P70351 Mouse | F1LZH3 Rat |
| Alternative names: | Enhancer of zeste homolog 1 (Drosophila) Enhancer of zeste homolog 1 ENX-2 ENX2 Ezh1 EZH1_HUMAN Histone-lysine N-methyltransferase EZH1 |
Images
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Fig1:
Western blot analysis of EZH1 on different lysates with Rabbit anti-EZH1 antibody (HA500184) at 1/1,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate (15 µg/Lane) Lane 2: Mouse hippocampus tissue lysate (30 µg/Lane) Lane 3: Rat brain tissue lysate (30 µg/Lane) Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500184, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 85 kDa Observed band size: 69-100 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.