Inhibin alpha Rabbit Polyclonal Antibody
cat.: HA500185
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Inhibin alpha aa 240-290. |
| Positive control: | Rat testis tissue lysate, HepG2 cell lysate, mouse testis tissue lysate, PANC-1 cell lysate, PANC-1. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:1000 1:50-1:100 |
| Uniprot #: | SwissProt: P05111 Human | Q04997 Mouse | P17490 Rat |
| Alternative names: | A inhibin subunit A inhibin subunit precursor AW55578 IHA inhA INHA_HUMAN Inhibin alpha chain Inhibin alpha chain precursor Inhibin alpha subunit |
Images
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Fig1:
Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody (HA500185) at 1/1,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Mouse ovary tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 135 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500185, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: multiple bands Observed band size: multiple bands |
|
Fig2: ICC staining of Inhibin alpha in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500185, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.