GABPA Rabbit Polyclonal Antibody
cat.: HA500187
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within human GABPA aa 1-200.
Positive control: A431 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q06546 Human | Q00422 Mouse
Alternative names: E4TF 1A E4TF1 60 E4TF1A GA binding protein alpha chain GA binding protein transcription factor alpha subunit 60kDa GA binding protein transcription factor alpha subunit GA-binding protein alpha chain GABP A GABP alpha subunit GABP subunit alpha Gabpa GABPA_HUMAN Human nuclear respiratory factor 2 subunit alpha NFT 2 NFT2 NRF 2 NRF 2A NRF2 NRF2A Nuclear respiratory factor 2 alpha subunit Nuclear respiratory factor 2 subunit alpha RCH04A07 Transcription factor E4TF1 60 Transcription factor E4TF1-60
Images
HA500187_1.jpg Fig1: Western blot analysis of GABPA on different lysates with Rabbit anti-GABPA antibody (HA500187) at 1/5,000 dilution.

Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: 293T cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 16 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500187) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.