| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TCF21 aa 125-175. |
| Positive control: | Hela cell lysate, 293T cell lysate, K562 cell lysate, human lung tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O43680 Human |
| Alternative names: | bHLHa23 Capsulin Class A basic helix-loop-helix protein 23 Epicardin POD 1 Pod-1 POD1 Podocyte expressed 1 Podocyte-expressed 1 TCF-21 tcf21 TCF21_HUMAN Transcription factor 21 |
|
Fig1:
Western blot analysis of TCF21 on k562 cell lysate with Rabbit anti-TCF21 antibody (HA500188) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500188, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/20,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 20 kDa |