LIM Kinase 1 Rabbit Polyclonal Antibody
cat.: HA500189
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 73 kDa
Isotype: IgG
Immunogen: Recombinant protein within human LIM Kinase 1 aa 450-647.
Positive control: Hela cell lysate, SKOV-3 cell lysate, mouse lung tissue lysate, rat stomach tissue lysate, rat brain tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysate, MCF-7, human liver carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue.
Subcellular location: Cytoskeleton, Nucleus, Cytoplasm, Lamellipodium.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P53667 Human | P53668 Mouse | P53669 Rat
Alternative names: EC 2.7.1.37 LIM domain containing protein kinase LIM domain kinase 1 LIM kinase LIM motif containing protein kinase LIMK 1 LIMK LIMK-1 limk1 LIMK1_HUMAN
Images
HA500189_1.jpg Fig1: Western blot analysis of LIM Kinase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500189, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: SKOV-3 cell lysate
HA500189_2.jpg Fig2: Western blot analysis of LIM Kinase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500189, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: Rat stomach tissue lysate
HA500189_3.jpg Fig3: Western blot analysis of LIM Kinase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500189, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: Rat skeletal muscle tissue lysate
HA500189_4.jpg Fig4: ICC staining of LIM Kinase 1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500189, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500189_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-LIM Kinase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500189, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500189_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-LIM Kinase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500189, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500189_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-LIM Kinase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500189, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500189_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-LIM Kinase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500189, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.