HA500190

MCT1 Rabbit Polyclonal Antibody

cat.: HA500190

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 54 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human MCT1 aa 450-500.
Positive control:	HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, K-562 cell lysate, human prostate tissue, human colon carcinoma tissue, human endometrium tissue, 293, A431.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:1,000 1:200 1:200-1:1,000 1:500-1:1,000
Uniprot #:	SwissProt: P53985 Human
Alternative names:	FLJ36745 HHF7 MCT 1 MCT MGC44475 Monocarboxylate transporter 1 Monocarboxylate transporter Monocarboxylate transporter isoform 1 Monocarboxylic acid transporter 1 MOT1_HUMAN Slc16a1 SLC16A1 protein Solute carrier family 16 (monocarboxylic acid transporters) member 1 Solute carrier family 16 member 1 (monocarboxylic acid transporter 1) Solute carrier family 16 member 1

Images

	Fig1: Western blot analysis of MCT1 on different lysates with Rabbit anti-MCT1 antibody (HA500190) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500190) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-MCT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500190, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MCT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500190, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-MCT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500190, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Flow cytometric analysis of MCT1 was done on 293 cells. The cells were stained with the primary antibody (HA500190, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 min at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
	Fig6: Immunocytochemistry analysis of A431 cells labeling MCT1 with Rabbit anti-MCT1 antibody (HA500190) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCT1 antibody (HA500190) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.