CTNNA3 Rabbit Polyclonal Antibody
cat.: HA500192
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 100/57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CTNNA3 aa 30-70. |
| Positive control: | NIH/3T3 cell lysates, Daudi cell lysates, mouse heart tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UI47 Human | Q65CL1 Mouse |
| Alternative names: | 4930429L08Rik 4933408A16 Alpha catenin like protein Alpha T catenin Alpha T-catenin Cadherin associated protein Cadherin-associated protein Catenin alpha 3 Catenin alpha-3 Catna3 CTNA3_HUMAN CTNNA3 MGC26194 MGC75041 OTTHUMP00000019684 OTTMUSP00000028119 OTTMUSP00000028120 RP11-433J16.1 VR22 |
Images
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Fig1: Western blot analysis of CTNNA3 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500192, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of CTNNA3 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500192, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTNNA3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500192, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.