CYP24A1 Rabbit Polyclonal Antibody
cat.: HA500196
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 50-250. |
| Positive control: | Rat bladder tissue lysates, mouse lung tissue lysates, mouse kidney tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:10,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q07973 Human | Q64441 Mouse | Q09128 Rat |
| Alternative names: | 1 25 @dihydroxyvitamin D3 24 hydroxylase 1 25 dihydroxyvitamin D(3) 24 hydroxylase 1 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial 24 OHase 24-OHase 25-dihydroxyvitamin D(3) 24-hydroxylase CP 24 CP24 CP24A_HUMAN CYP 24 CYP24 CYP24A1 Cytochrome P450 24A1 Cytochrome P450 24A1 mitochondrial cytochrome P450 CC24 Cytochrome P450 family 24 Cytochrome P450 family 24 subfamily A member 1 Cytochrome P450 family 24 subfamily A polypeptide 1 Cytochrome P450 subfamily XXIV Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) Cytochrome P450-CC24 EC 1.14.13.n4 Exo mitochondrial protein HCAI HCINF1 MGC126273 MGC126274 mitochondrial P450 CC24 Vitamin D 24 hydroxylase Vitamin D(3) 24 hydroxylase Vitamin D(3) 24-hydroxylase |
Images
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Fig1: Western blot analysis of CYP24A1 on rat bladder tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500196, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of CYP24A1 on mouse lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500196, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CYP24A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500196, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.