Collagen IV Rabbit Polyclonal Antibody
cat.: HA500197
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 161 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human Collagen IV. |
| Positive control: | MCF-7 cell lysate, SH-SY5Y cell lysate, SH-SY5Y. |
| Subcellular location: | Basement membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P02462 Human |
| Alternative names: | Collagen alpha-1(IV) chain Arresten COL4A1 Collagen IV |
Images
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Fig1:
Western blot analysis of Collagen IV on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500197, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: SH-SY5Y cell lysate |
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Fig2: ICC staining of Collagen IV in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500197, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.