NDE1 Rabbit Polyclonal Antibody
cat.: HA500198
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDE1 aa 1-200. |
| Positive control: | Rat brain tissue lysates, rat cerebellum tissue, human liver tissue, Hela. |
| Subcellular location: | Cytoskeleton, centrosome, spindle, kinetochore, cleavage furrow. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9NXR1 Human | Q9ES39 Rat |
| Alternative names: | FLJ20101 HOM TES 87 LIS1 interacting protein NUDE1 rat homolog LIS1 interacting protein NUDE1 LIS4 NDE 1 NDE1 NDE1_HUMAN Nuclear distribution gene E homolog 1 Nuclear distribution protein nudE homolog 1 NUDE 1 NudE NudE nuclear distribution gene E homolog 1 (A. nidulans) NudE nuclear distribution gene E homolog 1 NUDE1 |
Images
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Fig1: Western blot analysis of NDE1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500198, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NDE1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500198, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDE1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500198, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of Hela cells labeling NDE1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500198, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.