PFKFB2 Rabbit Polyclonal Antibody
cat.: HA500200
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PFKFB2 aa 455-505. |
| Positive control: | MCF7 cell lysate, Hep G2 cell lysate human thyroid tissue, human uterus tissue, mouse heart tissue. |
| Subcellular location: | Cytosol, Nucleoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: O60825 Human | P70265 Mouse |
| Alternative names: | 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 6-bisphosphatase 6-P2ase 2 6-P2ASE heart-type isozyme 6PF 2 K/Fru 2 6 P2ASE heart type isozyme 6PF-2-K/Fru-2 6PF-2-K/Fru-26-P2ase 2 6PF-2-K/Fru-26-P2ase heart-type isozyme F262_HUMAN Fructose 2 6 bisphosphatase cardiac isozyme Fructose-2 PFK 2/FBPase 2 PFK/FBPase 2 PFKFB cardiac PFKFB2 |
Images
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Fig1:
Western blot analysis of PFKFB2 on different lysates with Rabbit anti-PFKFB2 antibody (HA500200) at 1/1,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 300 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500200, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58.5 kDa Observed band size: 55 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500200, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500200, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PFKFB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500200, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.