PIP5K1A Rabbit Polyclonal Antibody
cat.: HA500201
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PIP5K1A aa 400-562. |
| Positive control: | 293T cell lysate, SiHa cell lysate, SK-Br-3 cell lysate, rat skeletal muscle tissue, human tonsil tissue, human liver tissue, mouse testis tissue, SiHa. |
| Subcellular location: | Cell membrane, Nucleus, Nucleus speckle, Cytoplasm, ruffle, lamellipodium. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q99755 Human | P70182 Mouse | D3ZSI8 Rat |
| Alternative names: | 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha Phosphatidylinositol 4 phosphate 5 kinase type I alpha Phosphatidylinositol-4-phosphate 5-kinase type I alpha Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha PI4P5K Ia PI51A_HUMAN PIP5K A PIP5K1-alpha Pip5k1a PIP5KIalpha PtdIns(4)P-5-kinase 1 alpha |
Images
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Fig1:
Western blot analysis of PIP5K1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500201, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: SiHa cell lysate Lane 3: SK-Br-3 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-PIP5K1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500201, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PIP5K1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500201, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PIP5K1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500201, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PIP5K1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500201, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of PIP5K1A was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500201, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.