Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IF-Cell, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 33 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human GOLPH3L aa 50-250. |
Positive control: | Hela cell lysate, Daudi cell lysate, MCF-7 cell lysate, SK-Br-3 cell lysate, rat ovary tissue lysate, rat large intestine tissue lysate, mouse colon tissue lysate, rat large intestine tissue, A549, Hela. |
Subcellular location: | Golgi stack membrane, trans-Golgi network membrane. |
Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:500-1:1,000 |
Uniprot #: | SwissProt: Q9H4A5 Human | Q8R088 Mouse | Q66H74 Rat |
Alternative names: | GLP3L_HUMAN Golgi phosphoprotein 3-like golph3l GPP34-related protein GPP34R |
Fig1:
Western blot analysis of GOLPH3L on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500205, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Daudi cell lysate Lane 3: MCF-7 cell lysate Lane 4: SK-Br-3 cell lysate Lane 5: Rat ovary tissue lysate |
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Fig2:
Western blot analysis of GOLPH3L on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500205, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat large intestine tissue lysate Lane 2: Mouse colon tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-GOLPH3L antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500205, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig4: ICC staining of GOLPH3L in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500205, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Fig5: Flow cytometric analysis of GOLPH3L was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500205, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |