GOLPH3L Rabbit Polyclonal Antibody
cat.: HA500205
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GOLPH3L aa 50-250. |
| Positive control: | MCF-7 cell lysates, Hela. |
| Subcellular location: | Golgi stack membrane, trans-Golgi network membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:50-1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9H4A5 Human | Q8R088 Mouse | Q66H74 Rat |
| Alternative names: | GLP3L_HUMAN Golgi phosphoprotein 3-like golph3l GPP34-related protein GPP34R |
Images
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Fig1:
Western blot analysis of GOLPH3L on MCF-7 cell lysate with Rabbit anti-GOLPH3L antibody (HA500205) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500205, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33 kDa Observed band size: 33 kDa |
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Fig2: ICC staining of GOLPH3L in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500205, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of GOLPH3L was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500205, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.