Cannabinoid Receptor I Rabbit Polyclonal Antibody
cat.: HA500206
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cannabinoid Receptor I aa 1-116. |
| Positive control: | SH-SY5Y cell lysate, rat brain tissue lysate, mouse spinal cord tissue lysate, Hela, rat bladder tissue. |
| Subcellular location: | Mitochondrion outer membrane, cell membrane, membrane raft, axon, presynapse. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: P21554 Human | P47746 Mouse | P20272 Rat |
| Alternative names: | CANN6 Cannabinoid receptor 1 CB-R CB1 CB1A CB1K5 CB1R Central cannabinoid receptor CNR CNR1 CNR1_HUMAN OTTHUMP00000016838 OTTHUMP00000214579 |
Images
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Fig1:
Western blot analysis of Cannabinoid Receptor I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500206, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse spinal cord tissue lysate |
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Fig2: ICC staining of Cannabinoid Receptor I in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500206, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Cannabinoid Receptor I antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500206, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.