CYP7A1 Rabbit Polyclonal Antibody
cat.: HA500207
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CYP7A1 aa 50-250. |
| Positive control: | LO2 cell lysate, Huh7 cell lysate, Hepa1-6 cell lysate, rat liver tissue lysate, SiHa, HeLa. |
| Subcellular location: | Endoplasmic reticulum membrane, Microsome membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:200 1:200-1:500 |
| Uniprot #: | SwissProt: P22680 Human | P18125 Rat |
| Alternative names: | Cholesterol 7 alpha hydroxylase Cholesterol 7 alpha monooxygenase Cholesterol 7-alpha-hydroxylase Cholesterol 7-alpha-monooxygenase CP7A CP7A1_HUMAN CYP 7 CYP7 CYP7A1 CYPVII Cytochrome P450 7A1 Cytochrome P450, family 7, subfamily A, polypeptide 1 Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 MGC126826 MGC138389 |
Images
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Fig1:
Western blot analysis of CYP7A1 on different lysates with Rabbit anti-CYP7A1 antibody (HA500207) at 1/1,000 dilution. Lane 1: LO2 cell lysate Lane 2: Huh7 cell lysate Lane 3: Hepa1-6 cell lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SiHa cells labeling CYP7A1 with Rabbit anti-CYP7A1 antibody (HA500207) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CYP7A1 antibody (HA500207) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Flow cytometric analysis of CYP7A1 was done on HeLa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500207, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.