NMDAR2B Rabbit Polyclonal Antibody
cat.: HA500208
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | predicated 166 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NMDAR2B aa 1100-1300 / 1484. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse hippocampus tissue lysate, A549, MG-63. |
| Subcellular location: | Late endosome, cytoskeleton, cell membrane, postsynaptic cell membrane, lysosome. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:100-1:500 |
| Uniprot #: | SwissProt: Q13224 Human | Q01097 Mouse | Q00960 Rat |
| Alternative names: | AW490526 EIEE27 Glutamate [NMDA] receptor subunit epsilon 2 Glutamate [NMDA] receptor subunit epsilon-2 Glutamate Receptor Ionotropic N Methyl D Aspartate 2B Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B Glutamate receptor ionotropic NMDA2B Glutamate receptor subunit epsilon 2 Glutamate receptor, ionotropic, NMDA2B (epsilon 2) GRIN 2B GRIN2B hNR 3 hNR3 MGC142178 MGC142180 MRD6 N methyl D asparate receptor channel subunit epsilon 2 N methyl D aspartate receptor subtype 2B N methyl D aspartate receptor subunit 2B N methyl D aspartate receptor subunit 3 N-methyl D-aspartate receptor subtype 2B N-methyl-D-aspartate receptor subunit 3 NMDA NR2B NMDA R2B NMDAR2B NMDE2 NMDE2_HUMAN NME2 NR2B NR3 GluN2B |
Images
|
Fig1:
Western blot analysis of NMDAR2B on different lysates with Rabbit anti-NMDAR2B antibody (HA500208) at 1/2,000 dilution. Lane 1:mouse brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500208, 1/2,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 166 kDa Observed band size: 166 kDa |
|
Fig2: ICC staining of NMDAR2B in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500208, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: Flow cytometric analysis of NMDAR2B was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500208, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.