NMDAR2B Rabbit Polyclonal Antibody
cat.: HA500208
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | predicated 166 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NMDAR2B aa 1100-1300 / 1484. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse hippocampus tissue lysate, A549, rat cerebellum tissue, MG-63. |
| Subcellular location: | Late endosome, cytoskeleton, cell membrane, postsynaptic cell membrane, lysosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:100 1:100-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: Q13224 Human | Q01097 Mouse | Q00960 Rat |
| Alternative names: | AW490526 EIEE27 Glutamate [NMDA] receptor subunit epsilon 2 Glutamate [NMDA] receptor subunit epsilon-2 Glutamate Receptor Ionotropic N Methyl D Aspartate 2B Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B Glutamate receptor ionotropic NMDA2B Glutamate receptor subunit epsilon 2 Glutamate receptor, ionotropic, NMDA2B (epsilon 2) GRIN 2B GRIN2B hNR 3 hNR3 MGC142178 MGC142180 MRD6 N methyl D asparate receptor channel subunit epsilon 2 N methyl D aspartate receptor subtype 2B N methyl D aspartate receptor subunit 2B N methyl D aspartate receptor subunit 3 N-methyl D-aspartate receptor subtype 2B N-methyl-D-aspartate receptor subunit 3 NMDA NR2B NMDA R2B NMDAR2B NMDE2 NMDE2_HUMAN NME2 NR2B NR3 GluN2B |
Images
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Fig1:
Western blot analysis of NMDAR2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500208, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate |
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Fig2: ICC staining of NMDAR2B in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500208, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-NMDAR2B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500208, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of NMDAR2B was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500208, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.