PLA2R Rabbit Polyclonal Antibody
cat.: HA500209
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Rabbit, Cow |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 153 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PLA2R: 1021-1100/1463. |
| Positive control: | Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:2,000 1:100-1:500 1:100-1:500 1:100-1:500 |
| Uniprot #: | SwissProt: Q13018 Human | Q62028 Mouse |
| Alternative names: | 180 kDa secretory phospholipase A2 receptor C-type lectin domain family 13 member C CLEC13C M type receptor M-type receptor Phospholipase A2 receptor 1 PLA2 R PLA2-R PLA2G1R PLA2IR PLA2R PLA2R_HUMAN PLA2R1 Soluble PLA2-R Soluble PLA2R Soluble secretory phospholipase A2 receptor |
Images
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Fig1:
Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-PLA2R (HA500209) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 153 kD Observed band size: 153 kD |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLA2R) Polyclonal Antibody, Unconjugated (HA500209) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.