HA500210

SREBP1 Rabbit Polyclonal Antibody

cat.: HA500210

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	122 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within N-terminal human SREBP1 from aa25-aa55.
Positive control:	HepG2 cell lysate, mouse heart tissue lysate, mouse liver tissue lysate, 293 cell lysate, Jurkat cell lysate, A549 cell lysate, Hela, human lung tissue, human placenta tissue.
Subcellular location:	Endoplasmic reticulum membrane, Golgi apparatus membrane, COPII-coated vesicle membrane; Nucleus.
Recommended Dilutions: WB IF-Cell IHC-P	1:500-1:2,000 1:50-1:100 1:100-1:500
Uniprot #:	SwissProt: P36956 Human \| Q9WTN3 Mouse
Alternative names:	ADD 1 bHLHd1 Class D basic helix-loop-helix protein 1 D630008H06 Processed sterol regulatory element-binding protein 1 SRBP1_HUMAN SREBF 1 SREBF1 SREBP 1 SREBP 1c SREBP-1 SREBP1 Sterol regulatory element binding protein 1 Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 Sterol regulatory element binding transcription factor 1 Sterol regulatory element-binding transcription factor 1

Images

	Fig1: Western blot analysis of SREBP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500210, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Mouse liver tissue lysate Lane 4: 293 cell lysate Lane 5: Jurkat cell lysate Lane 6: A549 cell lysate
	Fig2: ICC staining of SREBP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500210, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-SREBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500210, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SREBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500210, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SREBP1 antibody (HA500210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-SREBP1 antibody (HA500210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-SREBP1 antibody (HA500210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Flow cytometric analysis of HeLa cells labeling SREBP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500210, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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