Uroplakin III Rabbit Polyclonal Antibody
cat.: HA500212
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Uroplakin III aa 50-207. |
| Positive control: | Rat bladder tissue lysates, rat bladder tissue, Hela. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: O75631 Human | D3ZZ76 Rat |
| Alternative names: | MGC119178 OTTHUMP00000028951 UP III UP3a UPIII UPIIIA UPK 3 UPK 3A UPK3 UPK3A UPK3A_HUMAN Uroplakin 3A Uroplakin III Uroplakin-3a Uroplakin3A UroplakinIII |
Images
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Fig1:
Western blot analysis of Uroplakin III on rat bladder tissue lysates with Rabbit anti-Uroplakin III antibody (HA500212) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500212) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Uroplakin III antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500212, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of Hela cells labeling Uroplakin III. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500212, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.