CLCNKB Rabbit Polyclonal Antibody
cat.: HA500214
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 75 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human CLCNKB from aa520-aa687. |
| Positive control: | Mouse testis tissue lysate, rat testis tissue lysate, MCF-7 cell lysate, human kidney tissue, human seminoma tissue, MG-63, A549, A431, Hela. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:1,000 1:100-1:500 1:50-1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P51801 Human | Q9WUB6 Mouse | P51802 Rat |
| Alternative names: | Bartter syndrome type 3 Chloride channel Kb Chloride channel kidney B Chloride channel protein ClC-Kb Chloride channel voltage sensitive Kb ClC K2 ClC-K2 ClCK2 CLCKB CLCKB_HUMAN CLCNKB hClC Kb hClCKb MGC24087 OTTHUMP00000011120 OTTHUMP00000011121 RP11 5P18.8 |
Images
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Fig1:
Western blot analysis of CLCNKB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500214, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lane 3: MCF-7 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLCNKB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500214, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human seminoma tissue using anti-CLCNKB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500214, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of MG-63 cells labeling CLCNKB with Rabbit anti-CLCNKB antibody (HA500214) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CLCNKB antibody (HA500214) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5: Flow cytometric analysis of CLCNKB was done on A549 cells. The cells were stained with the primary antibody (HA500214, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 min at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Immunocytochemistry analysis of A431 cells labeling CLCNKB with Rabbit anti-CLCNKB antibody (HA500214) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CLCNKB antibody (HA500214) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig7:
Immunocytochemistry analysis of Hela cells labeling CLCNKB with Rabbit anti-CLCNKB antibody (HA500214) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CLCNKB antibody (HA500214) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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