KCNJ13/Kir7.1 Rabbit Polyclonal Antibody
cat.: HA500215
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human KCNJ13/Kir7.1 from aa 200-aa360. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, human small intestine tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O60928 Human | P86046 Mouse | O70617 Rat |
| Alternative names: | Inward rectifier potassium channel 13 Inward rectifier K(+) channel Kir7.1 Potassium channel, inwardly rectifying subfamily J member 13 KCNJ13 Kir7.1 |
Images
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Fig1:
Western blot analysis of KCNJ13/Kir7.1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500215, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-KCNJ13/Kir7.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500215, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.