KCNJ13/Kir7.1 Rabbit Polyclonal Antibody
cat.: HA500215
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human KCNJ13/Kir7.1 from aa 200-aa360. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, human small intestine tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O60928 Human | P86046 Mouse | O70617 Rat |
| Alternative names: | Inward rectifier potassium channel 13 Inward rectifier K(+) channel Kir7.1 Potassium channel, inwardly rectifying subfamily J member 13 KCNJ13 Kir7.1 |
Images
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Fig1:
Western blot analysis of KCNJ13/Kir7.1 on Mouse brain tissue lysate with Rabbit anti-KCNJ13/Kir7.1 antibody (HA500215) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500215, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
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Fig2:
Western blot analysis of KCNJ13/Kir7.1 on Rat brain tissue lysate with Rabbit anti-KCNJ13/Kir7.1 antibody (HA500215) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500215, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature |
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Fig3: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-KCNJ13/Kir7.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500215, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.