D4 GDI Rabbit Polyclonal Antibody
cat.: HA500217
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human D4 GDI aa 65-201. |
| Positive control: | A549 cell lysate, Raji cell lysate, RAW264.7 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human colon tissue, human tonsil tissue, Raji. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:250 1:1,000 |
| Uniprot #: | SwissProt: P52566 Human | Q61599 Mouse Entrez Gene: 362456 Rat |
| Alternative names: | Arhgdib D4 D4 GDP dissociation inhibitor GDIA 2 GDIA2 GDID 4 GDID4 GDIR2_HUMAN GDP dissociation inhibitor D4 LY GDI Ly-GDI LYGDI MGC108926 RAP1GN1 Rho GDI 2 Rho GDI beta Rho GDI2 Rho GDP dissociation inhibitor (GDI) beta Rho GDP dissociation inhibitor 2 Rho GDP dissociation inhibitor beta Rho GDP-dissociation inhibitor 2 Rho-GDI beta RhoGDI2 |
Images
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Fig1:
Western blot analysis of D4 GDI on different lysates with Rabbit anti-D4 GDI antibody (HA500217) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD D4 GDI cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500217) at 1/1,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of D4 GDI on different lysates with Rabbit anti-D4 GDI antibody (HA500217) at 1/1,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (40 µg/Lane) Lane 4: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500217) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of Raji cells labeling D4 GDI with Rabbit anti-D4 GDI antibody (HA500217) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-D4 GDI antibody (HA500217) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of Raji cells labeling D4 GDI. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500217, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.