HA500218

p53R2 Rabbit Polyclonal Antibody

cat.: HA500218

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 41 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within C-terminal human p53R2 from aa 200-aa351.
Positive control:	Rat skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, mouse cerebellum tissue lysate, human skeletal muscle tissue lysate, Daudi cell lysate, mouse colon tissue, rat cerebellum tissue, SH-SY5Y.
Subcellular location:	Cytoplasm, Nucleus.
Recommended Dilutions: WB IHC-P IF-Cell FC	1:500-1:2,000 1:50-1:500 1:50-1:100 1:1,000
Uniprot #:	SwissProt: Q7LG56 Human \| Q6PEE3 Mouse Entrez Gene: 299976 Rat
Alternative names:	DKFZp686M05248 MGC102856 MGC42116 MTDPS8A MTDPS8B p53 inducible ribonucleotide reductase small subunit 2 homolog p53 inducible ribonucleotide reductase small subunit 2 like protein P53 inducible ribonucleotide reductase small subunit 2 short form beta p53 R2 p53-inducible ribonucleotide reductase small subunit 2-like protein p53R2 Ribonucleoside diphosphate reductase M2 subunit B Ribonucleoside-diphosphate reductase subunit M2 B Ribonucleotide reductase M2 B (TP53 inducible) Ribonucleotide reductase M2 B Ribonucleotide reductase small subunit like 2 p53 inducible RIR2B_HUMAN RRM 2B RRM2B TP53 inducible ribonucleotide reductase M2 B TP53-inducible ribonucleotide reductase M2 B

Images

	Fig1: Western blot analysis of p53R2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500218, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat skeletal muscle tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Mouse cerebellum tissue lysate
	Fig2: Western blot analysis of p53R2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500218, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skeletal muscle tissue lysate Lane 2: Daudi cell lysate
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-p53R2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500218, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-p53R2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA500218, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig5: ICC staining of p53R2 in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500218, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Fig6: Flow cytometric analysis of SH-SY5Y cells labeling p53R2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500218, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.