Presenilin 1 / PS-1 Rabbit Polyclonal Antibody
cat.: HA500219
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Presenilin 1 aa 330-380. |
| Positive control: | HaCaT (Human immortalized keratinocyte) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, MEF (Mouse embryonic fibroblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C6 (Rat glioma cell) cell lysate, human small intestine tissue. |
| Subcellular location: | Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cell membrane, Early endosome, Early endosome membrane, Cytoplasmic granule, growth cone, neuron projection, axon, synapse. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:50-1:500 |
| Uniprot #: | SwissProt: P49768 Human | P49769 Mouse | P97887 Rat |
| Alternative names: | AD3 Ad3h FAD Homo Sapiens Clone CC44 Senilin 1 Presenilin-1 CTF12 Protein S182 PS 1 PS-1 PS1-CTF12 PSEN1 PSN1_HUMAN PSNL1 S182 |
Images
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Fig1:
Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA500219) at 1/5,000 dilution. Lane 1: HaCaT (Human immortalized keratinocyte) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 3: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 4: MEF (Mouse embryonic fibroblast) cell lysate cell lysate Lane 5: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 6: C6 (Rat glioma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500219, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Presenilin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500219, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.