Cytochrome b-c1 complex subunit 9/UQCR10 Rabbit Polyclonal Antibody
cat.: HA500222
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 7 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human UQCR10 from aa42-aa63 . |
| Positive control: | MCF-7 cell lysate, human skeletal muscle tissue lysate, Hela cell lysate, rat kidney tissue, human prostate carcinoma tissue, mouse kidney tissue, Hela. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9UDW1 Human | Q8R1I1 Mouse | B2RYX1 Rat |
| Alternative names: | Cytochrome c1 non heme 7 kDa protein Cytochrome C1 nonheme 7kDa protein HSPC051 HSPC119 HSPC151 QCR9 Ubiquinol cytochrome c reductase complex Ubiquinol cytochrome c reductase complex (7.2 kD) Ubiquinol cytochrome c reductase complex 7.2 kDa protein Ubiquinol cytochrome c reductase complex III subunit X 7.2kDa Ubiquinol cytochrome c reductase complex III subunit X UCCR7.2 UCRC UQCR10 |
Images
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Fig1:
Western blot analysis of Cytochrome b-c1 complex subunit 9/UQCR10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500222, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Human skeletal muscle tissue lysate Lane 3: Hela cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500222, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500222, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500222, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Cytochrome b-c1 complex subunit 9/UQCR10 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500222, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.