NC2 alpha Rabbit Polyclonal Antibody
cat.: HA500224
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NC2 alpha aa 150-200. |
| Positive control: | SW1990 cell lysate, HepG2 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q14919 Human | Q9D6N5 Mouse | A0JPP1 Rat |
| Alternative names: | DR1 associated corepressor DR1 associated protein 1 (negative cofactor 2 alpha) DR1 associated protein 1 DR1 associated protein1 Dr1-associated corepressor Dr1-associated protein 1 DRAP 1 Drap1 NC 2 alpha NC 2alpha NC2 alpha NC2-alpha NC2A_HUMAN Negative co-factor 2-alpha Negative cofactor 2 alpha Negative cofactor 2alpha |
Images
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Fig1:
Western blot analysis of NC2 alpha on different lysates with Rabbit anti-NC2 alpha antibody (HA500224) at 1/5,000 dilution. Lane 1: SW1990 cell lysate Lane 2: HepG2 cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 27 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500224) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.