NC2 alpha Rabbit Polyclonal Antibody
cat.: HA500224
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NC2 alpha aa 150-200. |
| Positive control: | SW1990 cell lysate, Jurkat cell lysate, SK-Br-3 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat testis tissue, human cervix tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:500 |
| Uniprot #: | SwissProt: Q14919 Human | Q9D6N5 Mouse | A0JPP1 Rat |
| Alternative names: | DR1 associated corepressor DR1 associated protein 1 (negative cofactor 2 alpha) DR1 associated protein 1 DR1 associated protein1 Dr1-associated corepressor Dr1-associated protein 1 DRAP 1 Drap1 NC 2 alpha NC 2alpha NC2 alpha NC2-alpha NC2A_HUMAN Negative co-factor 2-alpha Negative cofactor 2 alpha Negative cofactor 2alpha |
Images
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Fig1:
Western blot analysis of NC2 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500224, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW1990 cell lysate Lane 2: Jurkat cell lysate Lane 3: SK-Br-3 cell lysate |
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Fig2:
Western blot analysis of NC2 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500224, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NC2 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500224, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-NC2 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500224, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.