NCOR2/SMRT Rabbit Polyclonal Antibody
cat.: HA500226
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 274 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human NCOR2. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, MG-63. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9Y618 Human | Q9WU42 Mouse | D3ZXN9 Rat |
| Alternative names: | CTG 26 CTG repeat protein 26 CTG26 N CoR2 N-CoR2 NCoR 2 Ncor2 NCOR2_HUMAN Nuclear Receptor Co-Repressor 2 Nuclear receptor corepressor 2 retinoic-acid-receptor-associated corepressor Silencing Mediator for Retanoid and Thyroid Hormone Receptors Silencing mediator of retinoic acid and thyroid hormone receptor SMAP 270 SMAP270 SMRT SMRTE SMRTE tau T3 receptor associating factor T3 receptor-associating factor Thyroid retinoic acid receptor associated corepressor Thyroid- TNRC 14 TNRC14 TRAC 1 TRAC TRAC1 |
Images
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Fig1:
Western blot analysis of NCOR2/SMRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500226, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate |
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Fig2:
Flow cytometric analysis of MG-63 cells labeling NCOR2/SMRT. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500226, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.