SNW1 Rabbit Polyclonal Antibody
cat.: HA500227
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 61 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human SNW1 aa 1-200.
Positive control: PC-12 cell lysates, NIH/3T3 cell lysates, Jurkat cell lysates, human kidney tissue, mouse colon tissue, rat testis tissue, Hela, NIH/3T3.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000-1:5,000
1:400-1:1,000
1:50-1:100
1:100-1:500
Uniprot #: SwissProt: Q13573 Human | Q9CSN1 Mouse | D4A8G7 Rat
Alternative names: Bx 42 Bx42 Homolog of Drosophila BX42 MGC119379 NCOA 62 Nuclear protein SkiP Nuclear receptor coactivator 62 kD Nuclear receptor coactivator NCoA 62 Nuclear receptor coactivator NCoA62 Prp 45 Prp45 PRPF 45 PRPF45 Ski interacting protein SKIIP SKIP SNW 1 SNW domain containing 1 SNW domain containing protein 1 SNW1 SNW1 protein
Images
HA500227_1.jpg Fig1: Western blot analysis of SNW1 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500227, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500227_2.jpg Fig2: Western blot analysis of SNW1 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500227, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500227_3.jpg Fig3: Western blot analysis of SNW1 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500227, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA500227_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SNW1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500227, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500227_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SNW1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500227, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500227_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SNW1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500227, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500227_7.jpg Fig7: Immunocytochemistry analysis of Hela cells labeling SNW1 with Rabbit anti-SNW1 antibody (HA500227) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SNW1 antibody (HA500227) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
HA500227_8.jpg Fig8: Flow cytometric analysis of SNW1 was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500227, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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