SFT Rabbit Polyclonal Antibody
cat.: HA500229
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SFT aa 1-147. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat skeletal muscle tissue lysate, mouse liver tissue lysate, rat kidney tissue, human breast tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:400 |
| Uniprot #: | SwissProt: P51668 Human | P61080 Mouse | D3ZDK2 Rat |
| Alternative names: | E2(17)KB1 SFT Stimulator of Fe transport UB2D1_HUMAN UBC 4/5 UBC4/5 UBC4/5 homolog UBC4/5, S. cerevisiae, homolog of UBCH 5 UBCH 5A UbcH5 UBCH5A Ube2d1 Ubiquitin carrier protein Ubiquitin carrier protein D1 Ubiquitin conjugating enzyme E2 17 kDa 1 Ubiquitin conjugating enzyme E2 D1 Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) Ubiquitin conjugating enzyme E2D 1 Ubiquitin conjugating enzyme UBCH5A Ubiquitin protein ligase D1 Ubiquitin-conjugating enzyme E2 D1 Ubiquitin-conjugating enzyme E2(17)KB 1 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-protein ligase D1 |
Images
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Fig1:
Western blot analysis of SFT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500229, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Rat skeletal muscle tissue lysate Lane 5: Mouse liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.