SFT Rabbit Polyclonal Antibody
cat.: HA500229
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SFT aa 1-147. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse testis tissue lysate, rat skeletal muscle tissue lysate, rat kidney tissue, human breast tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:400 |
| Uniprot #: | SwissProt: P51668 Human | P61080 Mouse | D3ZDK2 Rat |
| Alternative names: | E2(17)KB1 SFT Stimulator of Fe transport UB2D1_HUMAN UBC 4/5 UBC4/5 UBC4/5 homolog UBC4/5, S. cerevisiae, homolog of UBCH 5 UBCH 5A UbcH5 UBCH5A Ube2d1 Ubiquitin carrier protein Ubiquitin carrier protein D1 Ubiquitin conjugating enzyme E2 17 kDa 1 Ubiquitin conjugating enzyme E2 D1 Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) Ubiquitin conjugating enzyme E2D 1 Ubiquitin conjugating enzyme UBCH5A Ubiquitin protein ligase D1 Ubiquitin-conjugating enzyme E2 D1 Ubiquitin-conjugating enzyme E2(17)KB 1 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-protein ligase D1 |
Images
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Fig1:
Western blot analysis of SFT on different lysates with Rabbit anti-SFT antibody (HA500229) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: Raji cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: Mouse liver tissue lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (20 µg/Lane) Predicted band size: 17 kDa Observed band size: 15 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500229) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SFT antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500229, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.